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Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing
Nano Letters  (IF12.262),  Pub Date : 2022-04-15, DOI: 10.1021/acs.nanolett.2c00415
Yungen Wu, Daizong Ji, Changhao Dai, Derong Kong, Yiheng Chen, Liqian Wang, Mingquan Guo, Yunqi Liu, Dacheng Wei

Accurate and population-scale screening technology is crucial in the control and prevention of COVID-19, such as pooled testing with high overall testing efficiency. Nevertheless, pooled testing faces challenges in sensitivity and specificity due to diluted targets and increased contaminations. Here, we develop a graphene field-effect transistor sensor modified with triple-probe tetrahedral DNA framework (TDF) dimers for 10-in-1 pooled testing of SARS-CoV-2 RNA. The synergy effect of triple probes as well as the special nanostructure achieve a higher binding affinity, faster response, and better specificity. The detectable concentration reaches 0.025–0.05 copy μL–1 in unamplified samples, lower than that of the reverse transcript-polymerase chain reaction. Without a requirement of nucleic-acid amplification, the sensors identify all of the 14 positive cases in 30 nasopharyngeal swabs within an average diagnosis time of 74 s. Unamplified 10-in-1 pooled testing enabled by the triple-probe TDF dimer sensor has great potential in the screening of COVID-19 and other epidemic diseases.