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Development and optimization of Lysis gene E as a counter-selection marker with high stringency
Biotechnology Journal  (IF5.726),  Pub Date : 2022-04-04, DOI: 10.1002/biot.202100423
Wei Chen, Ruyi Chen, Ling He, Xiaotong Wu

Seamless modification of bacterial chromosomes is widely performed in both theoretical and practical research. For this purpose, excellent counter-selection marker genes with high stringency are needed.