Find Paper, Faster
Example:10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis
Journal of Biomedical Optics  (IF3.17),  Pub Date : 2021-11-01, DOI: 10.1117/1.jbo.26.11.116002
Joshua Philippe Olorocisimo, Jeric Briones, Kiyotaka Sasagawa, Makito Haruta, Hironari Takehara, Hiroyuki Tashiro, Norihiro Ishida-Kitagawa, Yasumasa Bessho, Jun Ohta

Significance: Gene expression analysis is an important fundamental area of biomedical research. However, live gene expression imaging has proven challenging due to constraints in conventional optical devices and fluorescent reporters. Aim: Our aim is to develop smaller, more cost-effective, and versatile imaging capabilities compared with conventional devices. Bioluminescence reporter-based gene expression analysis was targeted due to its advantages over fluorescence-based imaging. Approach: We created a small compact imaging system using micro-CMOS image sensors (μCIS). The μCIS model had an improved pixel design and a patterned absorption filter array to detect the low light intensity of bioluminescence. Results: The device demonstrated lower dark current, lower temporal noise, and higher sensitivity compared with previous designs. The filter array enabled us to subtract dark current drift and attain a clearer light signal. These improvements allowed us to measure bioluminescence reporter-based gene expression in living mammalian cells. Conclusion: Using our μCIS system for bioluminescence imaging in the future, the device can be implanted in vivo for simultaneous gene expression imaging, behavioral analysis, and optogenetic modulation.