Find Paper, Faster
Example:10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness [Developmental Biology]
Proceedings of the National Academy of Sciences of the United States of America  (IF11.205),  Pub Date : 2021-11-09, DOI: 10.1073/pnas.2109381118
Marcela Nouzova, Marten J. Edwards, Veronika Michalkova, Cesar E. Ramirez, Marnie Ruiz, Maria Areiza, Matthew DeGennaro, Francisco Fernandez-Lima, René Feyereisen, Marek Jindra, Fernando G. Noriega

Methyl farnesoate (MF) plays hormonal regulatory roles in crustaceans. An epoxidated form of MF, known as juvenile hormone (JH), controls metamorphosis and stimulates reproduction in insects. To address the evolutionary significance of MF epoxidation, we generated mosquitoes completely lacking either of the two enzymes that catalyze the last steps of MF/JH biosynthesis and epoxidation, respectively: the JH acid methyltransferase (JHAMT) and the P450 epoxidase CYP15 (EPOX). jhamt−/− larvae lacking both MF and JH died at the onset of metamorphosis. Strikingly, epox−/− mutants, which synthesized MF but no JH, completed the entire life cycle. While epox−/− adults were fertile, the reproductive performance of both sexes was dramatically reduced. Our results suggest that although MF can substitute for the absence of JH in mosquitoes, it is with a significant fitness cost. We propose that MF can fulfill most roles of JH, but its epoxidation to JH was a key innovation providing insects with a reproductive advantage.