Accurate source identification is the first step of pollution control in environmental emergency management, especially in marine pollution events. Dissolved organic matter (DOM) absorption and fluorescence (excitation-emission matrices, EEMs) analyses were applied to trace contaminant sources for a pollution event that occurred along the coast of Laizhou Bay, Bohai Sea. Parallel factor analysis (PARAFAC) of the EEMs identified four fluorescent components: terrestrial humic-like (C1), tryptophan-like (C2), and a mixture of terrestrial and marine humic-like (C3) and tyrosine-like (C4) components. The relationships among C1 to C4 and quality indices indicated that the DOM originated from terrestrial input and biological activity. The EEMs-PARAFAC results accompanied by the optical characteristics of DOM and fingerprinting demonstrated that the marine pollution event occurred was from enterprise emissions. The numerical simulation confirmed the reliability of EEMs-PARAFAC modeling for DOM fingerprinting of pollution sources in polluted regions. This study provided a feasible method for source recognition in marine pollution events.