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Example:10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay
Journal of the American Chemical Society  (IF16.383),  Pub Date : 2021-10-08, DOI: 10.1021/jacs.1c06325
Derong Kong, Xuejun Wang, Chenjian Gu, Mingquan Guo, Yao Wang, Zhaolin Ai, Shen Zhang, Yiheng Chen, Wentao Liu, Yungen Wu, Changhao Dai, Qianying Guo, Di Qu, Zhaoqin Zhu, Youhua Xie, Yunqi Liu, Dacheng Wei

Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the requirements for wide-population screening due to time-consuming nucleic acid extraction and amplification procedures. Here, we report a direct nucleic acid assay by using a graphene field-effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes). The assay relies on Y-dual probes modified on g-FET simultaneously targeting ORF1ab and N genes of SARS-CoV-2 nucleic acid, enabling high a recognition ratio and a limit of detection (0.03 copy μL–1) 1–2 orders of magnitude lower than existing nucleic acid assays. The assay realizes the fastest nucleic acid testing (∼1 min) and achieves direct 5-in-1 pooled testing for the first time. Owing to its rapid, ultrasensitive, easily operated features as well as capability in pooled testing, it holds great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.