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Efficient enzymatic synthesis of L-ascorbyl palmitate using Candida antarctica lipase B-embedded metal-organic framework
Biotechnology Progress  (IF2.681),  Pub Date : 2021-10-02, DOI: 10.1002/btpr.3218
Xiao-Jian Zhang, Feng-Yu Qi, Jia-Mei Qi, Fei Yang, Jiang-Wei Shen, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng

The Candida antarctica lipase B (CALB) was embedded in the metal-organic framework, zeolitic imidazolate framework-8 (ZIF-8), and applied in the enzymatic synthesis of L-ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF-8 achieved the enzyme loading of 80 mg g−1 with 11.3 U g−1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L-ascorbic acid in a 10-batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L−1 h−1, which was higher than ever reported continuous ASP biosynthesis reactions.