Find Paper, Faster
Example:10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion
Developmental Cell  (IF12.27),  Pub Date : 2021-09-10, DOI: 10.1016/j.devcel.2021.08.015
Xuchao Lyu, Jia Wang, Jianqin Wang, Ye-Sheng Yin, Yun Zhu, Lin-Lin Li, Shuangru Huang, Shuang Peng, Boxin Xue, Rongyu Liao, Shi-Qiang Wang, Mian Long, Thorsten Wohland, Boon Tin Chua, Yujie Sun, Pilong Li, Xiao-Wei Chen, Li Xu, Peng Li

Membrane contact between intracellular organelles is important in mediating organelle communication. However, the assembly of molecular machinery at membrane contact site and its internal organization correlating with its functional activity remain unclear. Here, we demonstrate that a gel-like condensation of Cidec, a crucial protein for obesity development by facilitating lipid droplet (LD) fusion, occurs at the LD-LD contact site (LDCS) through phase separation. The homomeric interaction between the multivalent N terminus of Cidec is sufficient to promote its phase separation both in vivo and in vitro. Interestingly, Cidec condensation at LDCSs generates highly plastic and lipid-permeable fusion plates that are geometrically constrained by donor LDs. In addition, Cidec condensates are distributed unevenly in the fusion plate generating stochastic sub-compartments that may represent unique lipid passageways during LD fusion. We have thus uncovered the organization and functional significance of geometry-constrained Cidec phase separation in mediating LD fusion and lipid homeostasis.