Example：10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Single-cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin-driven circadian network The EMBO Journal (IF11.598), Pub Date : 2021-09-06, DOI: 10.15252/embj.2021108614 Emma L Morris, Andrew P Patton, Johanna E Chesham, Alastair Crisp, Antony Adamson, Michael H Hastings
Circadian rhythms in mammals are governed by the hypothalamic suprachiasmatic nucleus (SCN), in which 20,000 clock cells are connected together into a powerful time-keeping network. In the absence of network-level cellular interactions, the SCN fails as a clock. The topology and specific roles of its distinct cell populations (nodes) that direct network functions are, however, not understood. To characterise its component cells and network structure, we conducted single-cell sequencing of SCN organotypic slices and identified eleven distinct neuronal sub-populations across circadian day and night. We defined neuropeptidergic signalling axes between these nodes, and built neuropeptide-specific network topologies. This revealed their temporal plasticity, being up-regulated in circadian day. Through intersectional genetics and real-time imaging, we interrogated the contribution of the Prok2-ProkR2 neuropeptidergic axis to network-wide time-keeping. We showed that Prok2-ProkR2 signalling acts as a key regulator of SCN period and rhythmicity and contributes to defining the network-level properties that underpin robust circadian co-ordination. These results highlight the diverse and distinct contributions of neuropeptide-modulated communication of temporal information across the SCN.