Example：10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
High-Titer Production of Olivetolic Acid and Analogs in Engineered Fungal Host Using a Nonplant Biosynthetic Pathway ACS Synthetic Biology (IF5.11), Pub Date : 2021-08-20, DOI: 10.1021/acssynbio.1c00309 Ikechukwu C. Okorafor, Mengbin Chen, Yi Tang
The microbial synthesis of cannabinoids and related molecules requires access to the intermediate olivetolic acid (OA). Whereas plant enzymes have been explored for E. coli and yeast biosynthesis, moderate yields and shunt product formation are major hurdles. Here, based on the chemical logic to form 2,4-dihydroxybenzoate-containing natural products, we discovered a set of fungal tandem polyketide synthases that can produce OA and the related octanoyl-primed derivative sphaerophorolcarboxylic acid in high titers using the model organism Aspergillus nidulans. This new set of enzymes will enable new synthetic biology strategies to access microbial cannabinoids.