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Exosomes derived from ADSCs containing miR-378 promotes wound healing by targeting caspase-3
Journal of Biochemical and Molecular Toxicology  (IF3.642),  Pub Date : 2021-08-15, DOI: 10.1002/jbt.22881
Tianfeng Zheng, Wenjun Shao, Jun Tian

Pathological scars and chronic wounds caused by injury, aging, or surgery have always been important public health problems, and there is an urgent need to study the driving forces to find more effective treatments. In this study, we extracted and identified ADSCs exosomes and found that they have the ability to protect HaCat cells from oxidative damage, including promoting proliferation and migration and reducing apoptosis. Further studies determined that the expression of miR-378 was significantly enriched in exosomes. Studies have found that miR-378 mimic can produce protection similar to ADSCs-exo. However, when miR-378 inhibitors are used on ADSCs, the damage protection of the secreted exosomes disappears. This proves that miR-378 enriched in exosomes can improve HaCat's oxidative stress damage. Luciferase experiments show that this effect is achieved by targeting caspase-3. These results indicate that ADSCs play a protective role in wound healing by secreting miR-378-rich exosomes.