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Astaxanthin Attenuates Adiponectin, Calprotectin, miRNA222 and miRNA378 in Obesity induced by High-Fat Diet in Rats.
Current Pharmaceutical Biotechnology  (IF2.837),  Pub Date : 2021-08-09, DOI: 10.2174/1389201022666210810105804
Sylvia A Boshra

BACKGROUND Astaxanthin suppressed obesity in rats fed with high-fat diet(HFD) via the restriction of adipose tissue build-out, therefore, improving insulin sensitivity and inflammation. Metformin reduces insulin resistance and may reduce weight. AIM Investigation of the effects of astaxanthin and metformin in obesity prompted by a high-fat diet. OBJECTIVE The present article investigates the effects of astaxanthin and metformin in obesity prompted by a high-fat diet in rats through measuring miRNA222 and 378. MATERIALS The rats were classified into four classes containing ten albino rats each: Group I(Normal group): nourished with ordinary diet for 8weeks. Group II(Control positive): nourished with a high-fat diet for 8 weeks. Group III: nourished with astaxanthin(50mg/kg)(1/40 LD50) orally plus a high-fat diet for 8weeks. Group IV: nourished with metformin (500mg/kg) orally plus a high-fat diet for 8 weeks. METHODS Leptin, adiponectin, calprotectin and interleukin 6 (IL-6) were assessed by rat-specific ELISA kits. Tumor necrosis factor-alpha (TNF-α), miRNA222 and miRNA378 expressions were quantified by quantitative real-time PCR. RESULTS Astaxanthin and metformin have anti-obesity and antioxidant actions and significantly decreased the weight of the body, glucose, insulin, triglycerides, total cholesterol, triglycerides and leptin, as well as plasma calprotectin & IL-6 and increased HDL-C and adiponectin. The liver TNF-αgene expression, adipose tissue miRNA222 and miRNA378 expression were decreased compared to HFD control rats. DISCUSSION AND CONCLUSION Astaxanthin has regulated the aberrant expression of miRNA222 and 378 that may be related to hyperlipidemia and insulin resistance. Accordingly, astaxanthin deserves a clinical trial in the future due to its effects on miRNAs involved in obesity.