The objective of this study was to evaluate the quality of strawberries distributed in global markets. We obtained strawberries from Egypt, Mexico, the Republic of Korea, Spain, and the United States of America (US) being sold in Singapore, and compared them with Japanese strawberries (cv. Benihoppe) using instrumental analysis and sensory evaluation. Sensory evaluation was conducted by Singaporean and Japanese assessors. The results showed that the quality of strawberries in Mexico, Spain, and Egypt were relatively close, but the others were significantly different. The strawberries from the US and Japan had high total soluble solids (TSS) content, while those from Egypt, Mexico, Spain, and the US were firmer and had higher titratable acidity (TA). In the sensory evaluation, the assessors preferred strawberries with high total soluble solids (TSS) and low titratable acidity (TA). These findings suggest that varieties with high TSS and low TA are desirable for the global market. In addition, compared with the Japanese assessors, the Singaporean assessors tended to prefer strawberries with lower TA. These preferences may change depending on the eating habits of the region.