Identification and differentiation of quince cultivars and genotypes are more difficult than other members of the pomoideae sub-family due to the more limited range of traits of this species. This research was conducted to study the vegetative and reproductive traits of quince germplasm in the central regions of Iran and to identify important traits in the differentiation of these cultivars and genotypes. For this, 11 cultivars and genotypes collected from Isfahan province in the collection of the Agricultural Research Center were compared during 2018 to 2020 according to the National Guideline for Distinctness, Uniformity, and Stability (DUS) in quince. Fifty-five traits related to this guideline were considered along with 10 vegetative and fruit complementary traits. The results showed significant diversity in all plant organs and the general shape of the tree. Traits such as growth vigor, pubescence density, internode length, leaf dimensions, angle of leaf apex, flower size, petal arrangement, the undulation of petal margin, flowering time, fruit size, neck length, prominences of around the eyes, and fruit ripening time played an essential role in the differentiation of cultivars and genotypes. Cluster analysis divided cultivars and genotypes into four main groups. This grouping was able to divide the studied germplasm based on quantitative and qualitative characteristics as well as their collection location. NB4 and KVD2 genotypes are among the promising genotypes due to the quantitative and qualitative characteristics of the fruit.