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Study of surface activation markers on CD3−CD16+ NK cells and their correlation with clinical manifestations in children with infectious mononucleosis
Microbiology and Immunology  (IF1.955),  Pub Date : 2021-06-08, DOI: 10.1111/1348-0421.12925
Wanding Ye, Wan Zhang, Shixiong Wu, Mengquan Zhu, Zhiwei Xu

This study aimed to investigate the proportion of surface activation markers on natural killer (NK) cells in children with infectious mononucleosis (IM) and to explore its clinical relevance. A total of 17 children hospitalized with IM were included in this study as the experimental group. Meanwhile, healthy children matched for age and gender served as controls. First, we isolated peripheral blood mononuclear cells from children with IM and healthy children. Then, NK cell surface markers were stained with monoclonal antibodies and analyzed by flow cytometry. The results showed that the percentage of CD3CD16+ NK cells was higher in peripheral blood lymphocytes from children with IM than that from healthy children (t = −4.52, P < 0.05). And the expression of the surface activation markers CD69 and CD25 on CD3CD16+ NK cells was also higher in children with IM (t = −7.729, P < 0.05; t = −5.068, P < 0.05). There was a positive correlation between the percentage of CD3CD16+ NK cells in peripheral blood and the duration of fever in children with IM (r = 0.530, P < 0.05). Therefore, the proportion of NK cell subsets in children's peripheral blood changes in the acute phase of IM, suggesting that NK cells enhance their cytotoxicity and play a role in the control of infection in children with IM. Higher levels of CD3CD16+ NK cells and the association with disease progression suggest that these cells might be a useful index to help evaluate the disease course.