Example：10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel Micromachines (IF2.891), Pub Date : 2021-03-26, DOI: 10.3390/mi12040358 Ann-Kathrin Reichenwallner, Esma Vurmaz, Kristina Battis, Laura Handl, Helin Üstün, Tivadar Mach, Gabriele Hörnig, Jan Lipfert, Lukas Richter
We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts.