Example：10.1021/acsami.1c06204 or Chem. Rev., 2007, 107, 2411-2502
Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation Biotechnology Progress (IF2.681), Pub Date : 2021-03-26, DOI: 10.1002/btpr.3150 Dana Budde, Gian-Luca Albano, Thomas Noll, Elke Jurkiewicz
The presence of leachables in biopharmaceutical processes using single-use technologies (SUT) is well known. For the detection and quantification of the latter, extractable studies of SUT are very common nowadays. Although a mixture of compounds is regularly found in extractable studies, research has only been carried out regarding the effect of individual compounds on cell culture and the cumulative effect of a mix of leachables has not been investigated yet. In this study, a set of leachable model compounds (LMCs) was chosen and the effect of the LMCs on a Chinese hamster ovary DG44 cell line producing an IgG antibody was investigated concerning cell growth, cell cycle distribution and productivity. It was shown that even if worst-case concentrations were used, the LMCs solely impact cell growth. Additionally, interaction studies revealed that the inhibiting effect of the mix is lower than the expected cumulative effect. A strong antagonism between the antioxidant butylated hydroxytoluene and the plasticizer Tris(2-ethylhexyl)trimellitate was found using an isobologram analysis.