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Isolation, Purification, and Characterization of a Lectin from the Fungus Fusarium solani 4
Applied Biochemistry and Microbiology  (IF0.886),  Pub Date : 2021-03-12, DOI: 10.1134/s0003683821020095
R. S. Mukhammadiev, R. S. Mukhammadiev, E. V. Skvortsov, L. R. Valiullin, A. P. Glinushkin, T. V. Bagaeva

Abstract

Electrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-Na electrophoresis in the presence of DDS-Na equal to 19 kDa. The physicochemical properties and carbohydrate specificity of the lectin, as well as its in vitro effect on mammalian cancer cells, were studied. The results opened new prospects for the further study of lectin and its potential applications as a medical agent.