Leaf spot disease caused by several phytopathogenic fungi is the most common problem in the solanaceous crops. A total of 36 leaf-spot-associated fungal species were isolated from brinjal, tomato, and chilli crops, which were identified by rDNA sequencing of the internal transcribed spacer region. These fungal species belonged to eight genera (Aspergillus spp, Alternaria spp, Sarocladium spp, Nigrospora spp, Corynespora spp, Chaetomium spp, Penicillium spp, and Fusarium spp). The results of the pathogenicity test of the isolates revealed that eight isolates had similar characteristics even in cross-inoculation with brinjal, tomato, and chilli crops. Twenty-two isolates showed pathogenicity in their respective hosts and cross-pathogenicity of any one of the plants tested. The results suggest that periodic monitoring of leaf spot pathogen distribution in the field can shed light on tentative emergence of new diseases.